Developing tools for multiplex to single EV analysis pipelines

Due to the small size of the majority of EVs, phenotyping methods have remained limited in sensitivity and scale. While emerging technologies are becoming capable of detecting tens of molecules on the surface of a particle, these sensitivity limits are generally confined to a small number of fluorophores. These emerging technologies are therefore limited in the scale of their phenotypic capabilities and ideally require high-throughput, scalable, multi-parameter methods to provide informed choices of marker selection before use. The Translational Nanobiology Section of the Center for Cancer Research Laboratory of Pathology at NIH has been developing tools to implement a multiplex to single EV analysis pipeline, enabling screening of hundreds of EV phenotypes through stitched-multiplex analysis and using the data to inform high-sensitivity single-EV analysis methods. Methods for utilizing these tools with the Cytek Aurora will be demonstrated.

Speaker: Joshua Welsh, PhD, University of Southampton, UK
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