Preparative electrophoresis systems for targeted size selection of biomolecules.
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How it works:
>Intact cells or nuclei are loaded into SageHLS cassettes.
>Electrophoretic extraction leaves chromosomal-length genomic DNA immobilized in the sample well wall.
>Custom Cas9 cleavases perform enzymatic DNA processing.
>Size selection electrophoresis moves Cas9-cleaved target regions into the gel channel, away from uncut untargeted genomic DNA in the sample well.
>Products are localized by qPCR after electroelution from the gel channel.
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Related technologies: Targeted DNA/protein size collection
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Preparative electrophoresis systems for targeted size selection of biomolecules.
More info at:
www.sagescience.com/