Coy Labs produces Glove Boxes, Anaerobic Chambers, and controlled atmosphere glove boxes.
Automated microscopy and Spatial Proteomics
Real-time, label free cell analysis
Nano and micro particle analysis
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Theranostics: From Mice to Men and Back
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We decided to do a deep dive into Range+T to get a better handle the method, and to develop best practices for using...
April 2024 publication revealing benefits of using intravital microscopy in trascriptomics studies
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Transcriptional activation of Bmal1 drives the inflammatory activity of monocytes by modulating mitochondrial unfolded...
Minimal information for studies of extracellular vesicles (MISEV2023)
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Omics Studies of Tumor Cells under Microgravity Conditions
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It is important for the analysis of data from space experiments to distinguish unspecific stress reactions from...
Hypoxia activates constitutive Luciferase reporter constructs
Jul 13, 2017
Studies in our laboratory sought to examine the hypoxic regulation of HIF activity in placental trophoblast cells, through the use of dual-reporter luciferase assays. Our study demonstrates that hypoxic conditions cause a significant increase in the level of constitutive luciferase reporter activity. We also show that this induction is not a cell-type or species-specific phenomenon and provides an alternative method for normalizing transfection efficiency in luciferase assays under hypoxic conditions. Our results suggest that in studies dealing with hypoxic conditions, caution should be used when interpreting measurements of transcriptional activity by traditional dual-reporter assays.
Related technologies: Hypoxia and O2 controlled environment isolators | Anaerobic boxes
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