Mounting evidence implicated the classical complement pathway (CP) in normal brain development, and the pathogenesis of neuropsychiatric and neurodegenerative diseases using MERFISH technology. 

MERFISH integrates spatial transcriptomics technology with high resolution spatial imaging, In this webinar we will discuss the methods used to acquire spatial information about human spinal cord development.

In this webinar Prof. Sandro Santagata, will reveal how Orion high-plex imaging and the use of this data, is valuable to identify cell lineages and states (molecular phenotypes) and map their spatial organization and interactions at different scales.

Samples from 82 patients with NSCLC and matched pre- and post-ICI biopsies were subjected to genomic profiling and to whole-slide biomarker quantitation at subcellular resolution using the Orion™ platform from RareCyte. 

Unlike flow cytometers, the CellVoyager CQ1 confocal quantitative image cytometer does not require pretreatment such as detachment, and can easily perform 3D analysis and time-lapse 3D analysis of cell aggregates such as spheroids.

Orion Reagents are tailored to enable spatial biomarker quantitation rapidly, easily and with maximum flexibility. Choose from a wide range of rigorously validated pre-made panels and labelled antibodies to get started immediately on Orion system.

In this webinar, Dr. McArdle describes how multiplex immunostaining is a key method for understanding spatial context of the tissue microenvironment and the statistical quantification of cells. She also presents examples of her open-source software