“We used Curiox HT2000 to stain T lymphocytes (including Treg) from peripheral blood cells. We tested different antibodies for both surface and intracellular markers.The laminar flow cell washing procedure was found to be much more gentle than centrifugation, therefore the proportion of intact lymphocytes was higher compared to standard centrifugation. Additionally, the sample preparation is much faster and easier using Curiox laminar wash technology than classical centrifugation.”