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“We plan to use the Pippin prep for our NGS library purification.”

Dr. Jiri Baloun

CEITEC – Central European Institute of Technology, Brno, Czech Republic

CEITEC – Central European Institute of Technology, Brno, Czech Republic

We use the Pippin prep for size selection of different types of NGS libraries.

Mgr. Gabriela Šrámková

Charles University, Faculty of Science, Prague, Czech Republic

Charles University, Faculty of Science, Prague, Czech Republic

Pippin Prep – Sage Science

Targeted Size Selection for Next-Gen Sequencing

The Pippin Systems facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The current high-throughput DNA sequencers — including platforms from Illumina, Ion Torrent, and 454 – require tightly sized insert libraries for optimum sequencing performance. The platform features the ability to collect narrow and even fragment distributions, without contamination. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer.

Pippin Prep automatically collects DNA fragments in range from 90bp – 1.5kb.

The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination.

Benefits of the Pippin Prep System:

  • Saves time and effort compared to manual gel purification
  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis

Key Downstream Technologies:

  • Paired-end sequencing
  • emPCR
  • RNA-seq
  • ChIP-seq

More info at: http://www.sagescience.com/products/pippin-prep/

Regina Fillerová

Field application specialist

Regina Fillerová

+420 731 127 718

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