Minimal information for studies of extracellular vesicles (MISEV2023)

Summary for Size Exclusion Chromatography:
SEC is an easily accessible technique for size-based separation of particles.
Columns can be packed with a variety of matrices and at different scales, depending on desired capacity and resolution of separation.
Reporting recommendations:
– type of matrix and pore size;
– height and diameter (or volume) of matrix-containing column;
– method of column packing (or source of commercially-available columns);
– source, volume and particle concentration of pre-SEC sample, including any prior separation/concentration steps;
– buffer composition;
– specify gravity flow or pressure. If pressure, indicate pump system and pressure parameters;
– void volume and numbers and volume of fractions collected;
– any post-SEC concentration methods;
– if columns are re-used, method of column regeneration and number of times the column has been used.

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