Non-invasive real-time pulsed Doppler assessment of blood flow in mouse ophthalmic artery

The method we present allows a non-invasive assessment of blood flow parameters in the OphA at its point of emergence from the cranium and into the orbit. This approach enables longitudinal experiments in the same animal and eliminates the need for invasive procedures to assess the OphA in mice. The high time resolution, accuracy, and reproducibility of the presented approach, combined with reduced data variability, enable the characterization of the kinetics of drug responses with previously unmatched temporal resolution.
Highlights

• Non-invasive Doppler ultrasound for measurement of blood flow velocity in mouse OphA
• Real-time and high-resolution measurements for acute or longitudinal studies
• Functional validation of method with pharmacological and surgical manipulations
• Application to blood flow regulatory mechanisms or pharmacological screening studies

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