Meet ACCELA at the “Analytical Cytometry XIII” conference, October 4–7, 2025, Mikulov

Come visit our spacious and welcoming booth, where we’ll be showcasing the latest innovations and hands-on workshops in the world of “flow”, including: Spectral Flow Cytometry, Spectral Sorting, Gentle & Image-Guided Cell Sorting, Nano Flow Cytometry…and much more. Whether you’re looking to explore the latest tech, connect with fellow scientists, or dive deep into educational sessions, this is your chance.
Let’s push the boundaries of cytometry together.

1) Introducing Cytek Aurora Evo, the New Standard of Full Spectrum Cytometry

The Cytek Aurora Evo system is the latest advancement in spectral flow cytometry. Built on the trusted foundation of the Cytek Aurora and Northern Lights systems, the Cytek Aurora Evo system has been reengineered to carry forward the proven reliability and reproducibility of the Cytek Aurora system, while introducing high-throughput and automation capabilities. As pioneers in spectral flow cytometry, Cytek remains at the forefront of innovation with our proprietary Full Spectrum Profiling (FSP) technology.

2) Elevate you Spectral sorting experience for small and large particles with Cytek Aurora CS.

The Cytek Aurora CS system provides remarkable flexibility, enabling the use of a wide array of new fluorochrome combinations without requiring the reconfiguration of the detectors. Sharing the same optical system as the Cytek Aurora analyzer allows for the easy transfer of experiments from the analyzer to the Aurora CS system without the need to modify your panels. The state-of-the-art optics and low-noise electronics ensure high resolution and high sensitivity for identifying and isolating rare cell populations deep into the gating hierarchy. The result is a system that delivers exceptional performance at the single-cell level which can resolve the most challenging cell populations, such as cells with high autofluorescence or low expression of key biomarkers, regardless of assay complexity. Equipped with advanced sorting features and monitoring tools, the Cytek Aurora CS system can isolate viable and functional cells for downstream studies.

3) From IDEAS to Intelligence: AI-Enhanced ImageStreamx data analysis

The ImageStream system is a multispectral imaging flow cytometer that captures high-resolution images of cells at high throughput. Paired with the powerful IDEAS image analysis software, it enables objective quantification of hundreds of cellular features – not only based on fluorescence intensity, but also on morphology, probe localization, and co-localization. To support advanced analysis needs, the software now integrates both Machine Learning and Deep Learning, providing more automated and scalable solutions for data interpretation. In this workshop, we will demonstrate how Artificial Intelligence (AI) enhances the objectivity and efficiency of image-based cytometric analysis. Examples will include:

4) Shedding Light on Extracellular Vesicles with Imaging Flow Cytometry

Accurate quantification and characterization of EVs remain challenging due to their small size -exosomes typically range from 30 to 100 nm in diameter. Conventional PMT-based flow cytometry also struggles with EV detection, hindered by both size limitations and the low refractive index of these vesicles. To address these challenges, we have adopted the Cytek Amnis ImageStream imaging flow cytometry platform, which combines the statistical power of flow cytometry with the enhanced resolution of microscopy. Its time-delay integration (TDI) imaging system provides increased sensitivity for detecting small particles. In this workshop, we will demonstrate the application of the ImageStream technology for EV immunophenotyping and for analyzing their uptake by immune cells. We will also discuss strategies to optimize EV detection and analysis.

5) Introducing Cytek Aurora Evo, hands-on experience on a smarter & faster Full Spectrum Flow Cytometer

The Cytek Aurora Evo system is the latest advancement in spectral flow cytometry. Built on the trusted foundation of the Cytek Aurora and Northern Lights systems, the Cytek Aurora Evo system has been reengineered to carry forward the proven reliability and reproducibility of the Cytek Aurora system, while introducing high-throughput and automation capabilities. High-Throughput: Sample flow rates up to 200 µL/min using both tubes and plates without sacrificing resolution.

6) Hands-on cell sorting session: Easily sort large and fragile cells with WOLF Gentle Cell Sorter

The WOLF Cell Sorter was designed and created by a team of scientists and engineers who wanted to solve a classic challenge in biological research: how to sort cells of high quality effectively and easily. NanoCellect has created an entirely new type of microfluidic cell sorter that extends modern flow cytometry methods. By using proven detection technologies, NanoCellect focuses on innovative cartridge technology (commonly known as a “microfluidic chip”) and accessible, intuitive software that makes the operation simple and safe for researchers and their samples. The WOLF benchtop flow cytometry cell sorter allows for the gentle separation of cell types in bulk sorting and the isolation and dispensing of individual cells into plates. Single-cell sorting is completed in 96- or 384-well plates when using the WOLF with the N1 Single Cell Dispenser. This flexibility and performance allows users to complete sorting experiments across different research fields and application areas like single-cell genomics, cell line development, gene editing, antibody discovery, stem cell cloning, genomic sample prep, and more.

7) Introducing VERLO: Image-Guided & Gentle Cell Sorter

VERLO is providing conventional flow cytometry and cell sorting along with the ability to perform imaging analysis to identify and sort cells based on morphology, subcellular localization and more. The VERLO instrument expands the capabilities of gentle benchtop microfluidic cell sorting. With two lasers and nine colors, plus 3 label-free parameters, it maintains simple workflows for either bulk sorting or single-cell dispensing into 96- or 384-well plates with an integrated cell dispensing and sample cooling.
Spatial Information: Captures images of each cell. In addition to fluorescence intensity, the VERLO provides spatial information such as cell morphology and marker localization.

8) Precise and efficient Extracellular Vesicles purity detection with ExoPlorer Nano Flow Cytometer

The Exoplorer Nano-Flow Cytometer redefines nanoparticle analysis as the all-in-one solution for revealing nanoscale secrets with groundbreaking precision. Combining superior sensitivity, resolution, and throughput, this instrument delivers comprehensive insights across the full 5‒3000 nm spectrum, enabling simultaneous size distribution mapping, bead-free absolute counting, and multi-parameter biochemical profiling. In this study we underline the fact that reliable measurements of extracellular vescicles (EV) concentrations are essential for biomarker research. Moreover, different instruments measures different sample concentrations. Using ExoPlorer nano flow cytometer we have been able to accurately measure EVs concentration and size.

9) Nano Flow Cytometry session: The Critical Role of Refractive Index Settings in Light Scattering-based Particle Sizing.

Nanoflow cytometry has been widely applied for size measurement of extracellular vesicles (EVs) and other nanoparticles due to its high throughput and single-particle resolution. The technique estimates particle size based on light scattering signals, where the Refractive Index (RI) of particles plays a critical role in determining measurement accuracy. Using ExoPlorer Nano Flow Cytometer, we analyzed the same standard bead sample using different RI assumptions to systematically evaluate how RI selection affects particle size readouts. The results demonstrate that variations in RI settings significantly influence the measured size distribution and related statistical parameters. This approach highlights the importance of appropriate RI parameter selection in Nano Flow Cytometry to ensure accuracy and comparability in nanoparticle sizing.

We can’t wait to meet you, The ACCELA Team